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SRX21829821: AROF
1 ILLUMINA (Illumina HiSeq 1500) run: 3.7M spots, 1.1G bases, 327.1Mb downloads

Design: Base-edited cell cultures of three biological replicates were mixed with an equal proportion and genomic DNAs were extracted. DNA fragments containing the target region (200-250 bp) were amplified from the extracted genomic DNAs by PCR. NEBNext Ultra DNA Library Prep Kit was used to convert the amplicon into indexed libraries for NGS on the Illumina platform.
Submitted by: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
Study: sequencing the RBS variants generated by base editing
PRJNA1019141 • SRP461899 • All experiments • All runs
show Abstracthide Abstract
In this study, we propose utilizing the BETTER base editing method to edit the ribosome binding site (RBS) in front of key enzymes within the phenylalanine biosynthetic pathway, generating an RBS mutation library.
Sample:
SAMN37475059 • SRS18929185 • All experiments • All runs
Organism: Corynebacterium glutamicum
Library:
Name: RBS2
Instrument: Illumina HiSeq 1500
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 3.7M spots, 1.1G bases, 327.1Mb
Run# of Spots# of BasesSizePublished
SRR261159853,658,6831.1G327.1Mb2023-09-20

ID:
29645366

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